Evaluation of Trypanosoma cruzi 30dH biotherapic: an in vitro study

Abstract

In the present study, our main objective was to evaluate the biological activity and the kinetics of the humoral immune response in Swiss Webster mice using the biotherapic agent Trypanosoma cruzi 30DH.This compound was prepared following the pharmacotechnique described by Roberto Costa. Trypanosoma cruzi 30DH was evaluated by the parasitemia parameters and the humoral immune response, performed by Indirect Immunefluorescence Reaction (RIFI) to analyse IgM and IgG antibodies. Based on the biological activity, 50% of the animals in the group treated with the biotherapic agent survived. The group which received treatment and infection simutaneously and in the control group had 100% mortality. Higher IgG levels in the group of animals previously treated with the compound could be observed, presenting 1:80 and with sub-patent parasitemia. Our results suggest that the previous treatment with the biotherapic agent showed a humoral immune response, with higher serological titers and absence of parasites in the blood.