The Association of Ponderal Benznidazole with its Ultra-high Diluted Formula Reduces the Toxic Effects and Allows Increasing of Dose in Dose-dependent Protocol in Mice Infected with Trypanosoma cruzi

  • Denise Lessa Aleixo
  • Mateus Joacir Benvenutti Universidade Estadual de Maringá
  • Katiucha Rebeca Jennifer Lopes Lera Faculdades Ingá
  • Larissa Ciupa Universidade Estadual de Maringá
  • Fabiana Nabarro Ferraz Universidade Estadual de Maringá
  • Silvana Marques de Araújo Universidade Estadual de Maringá
Keywords: Trypanosoma cruzi, ultra-high dilution, homeopathy, benznidazole, parasitemia, toxic symptoms


Although several diseases are treated by toxic drugs, their side effects may hamper adherence to the therapy. The aim of this study is to evaluate the effect of the association of ponderal benznidazole (BZ) with its ultra-high diluted (UHD) formula on clinical and parasitological parameters of mice infected by Trypanosoma cruzi (T. cruzi). 24 non-isogenic Swiss mice were divided into groups: CI – infected animals treated with 7% alcohol; BZp – infected animals treated with BZ (500 mg/ kg) from the beginning of infection; BZp+d – infected animals treated with ponderal BZ and with UHD BZ, which started to be administered four days after the beginning of treatment with ponderal BZ; CNI - group of non-treated and non-infected animals. The UHD medicine was prepared according to Phamacopoeia until 30x. The different treatment schedules were statistically compared through parasitological and clinical parameters. The group BZp+d displayed more favorable clinical evolution than the group BZp, with improvement of mass gain, feed conversion and water intake, presenting data approximated to CNI group. The significant increase of the body temperature of BZp+d group indicates an activation of the immune system which was not observed in the other groups. Moreover, the anti-parasitic effect of the ponderal drug was maintained in all parasitological parameters of this group. By reducing the side effects and maintaining the action of the ponderal drug, the combination of toxic drugs with their UHD formula could be considered a way of improving efficacy of the treatment.

Author Biography

Denise Lessa Aleixo
Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá - Paraná, Brazil.
Basic Research (Biology)