Intoxication of wheat seedlings with 50 mM NaCl and follow-up attempt to â€œcureâ€ by extremely diluted NaCl (30cH)
Keywords:homeopathy, wheat stalk growth, NaCl, salt, high dilution
AbstractBackground: â€œHigh level of salinity deteriorates seed germination, growth and yield of crops in cultivated lands all over the world. There is no effective remedy to mitigate this global problemâ€, Mondal, Sukul et al.1 summed up the background of their study that led to the conclusion that homeopathically prepared, extremely diluted Natrum muriaticum (NaCl) 200cH promotes seed germination in seedlings of cowpea under salt stress.1 Aim: To investigate if the wheat germination model standardized at the Interuniversity College2,3 can be useful in investigating the NaCl issue; i.e. the study was not designed as a replication of the study by Mondal, Sukul et al.1 Methods: Grains of winter wheat (Triticum aestivum) were observed under the influence of extremely diluted NaCl (10-60) prepared by stepwise dilution and agitation according to a protocol derived from homeopathy (â€œN30câ€). Analogously prepared water was used for control (â€œW30câ€). Grains (500 per group) were pretreated (intoxicated) with (non-agitated) NaCl 50mM (â€œNâ€) or with water (â€œWâ€) prior to treatment with N30c or W30c. Seedlings were allowed to develop under standardized conditions for 32 h; germination of stalks and of roots were monitored at intervals of 4 h. Results: With regard to intoxication, the groups treated with N exhibited less growth than the W control groups. With regard to the attempt to â€œcureâ€, the N+N30c group was not statistically different from the N+W30c group, and the W+N30c group was not statistically different from the W+W30c group. Conclusion: The hypothesis that treatment of wheat seedlings with the extreme dilution NaCl 30cH will â€œcureâ€ the effect of previous intoxication with NaCl 50 mM on seed germination could not be accepted. If further experiments are to be performed on the wheat model, these should include different intoxication solutions (e.g. 100mM1), different treatment dilutions (e.g. 200cH1) or different durations of the experiment3. The use of cowpea1 instead of wheat should also be considered.
Copyright (c) 2021 P.C. Endler, Pia Lamest, Elke Liebig
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