Biological activity of interferon gamma and lipopolysaccharide on the nitric oxide production in C6 astroglioma cells and some unexpected effects of potentization

Authors

  • Clara Bonafini
  • Marta Marzotto
  • Debora Olioso
  • Paolo Bellavite

DOI:

https://doi.org/10.51910/ijhdr.v14i2.779

Keywords:

interferon gamma, potentization, succussion, high dilution

Abstract

Background: A proinflammatory environment is a hallmark of several neurodegenerative diseases where astrocyte involvement is also well established. Astrocytes and microglia in central nervous system are mainly involved in the release of cytokines, oxygen free radicals and nitric oxide (NO). Several studies on C6 astroglioma cells, a widely used in vitro model for these events, demonstrated that co-stimulation of this cell line with bacterial lipopolysaccharide (LPS) and interferon gamma (IFN-ï§) induces a synergistic nitric oxide synthase (iNOS) expression.1 In our laboratory we are using this versatile cell model in order to carefully investigate dose-response effects of various putative agonists or inhibitors and to assess the possible changes provoked in those agents by different procedures of dilution and succussion (agitation) (potentization according to the homeopathic terminology).

Author Biographies

Clara Bonafini

Department of Pathology and Diagnostics, University of Verona

Marta Marzotto

Department of Pathology and Diagnostics, University of Verona

Debora Olioso

Department of Pathology and Diagnostics, University of Verona

Paolo Bellavite

Drof. of General Pathology University of Verona (I)

Downloads

Published

2021-08-27

Most read articles by the same author(s)

1 2 > >>